製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NBL1
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from C-terminus of human NBL1.

  • ポジティブ・コントロール
    • Human lung carcinoma tissue, HeLa cells, extracts from Jurkat and COLO cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 関連性
    NBL1 (Neuroblastoma, suppression of tumorigenicity 1) is a candidate tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. NBL1 is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. It is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • D1S1733E antibody
    • DAN antibody
    • DAN domain family member 1 antibody
    • DAND 1 antibody
    • DAND1 antibody
    • Differential screening selected gene aberrant in neuroblastoma antibody
    • MGC8972 antibody
    • NB antibody
    • NBL 1 antibody
    • Neuroblastoma candidate region suppression of tumorigenicity 1 antibody
    • Neuroblastoma suppression of tumorigenicity 1 antibody
    • Neuroblastoma suppressor of tumorigenicity 1 antibody
    • NO 3 antibody
    • NO3 antibody
    • Zinc finger protein DAN antibody
    see all

画像

  • HeLa cells with ab70066 at 1/500 dilution, with and without the immunising peptide.
  • Human lung carcinoma tissue with ab70066 at 1/50 dilution, with and without the immunising peptide.
  • All lanes : Anti-NBL1 antibody (ab70066) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COLO cell extract
    Lane 3 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 19 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Fessel JP  et al. Interaction between bone morphogenetic protein receptor type 2 and estrogenic compounds in pulmonary arterial hypertension. Pulm Circ 3:564-77 (2013). WB ; Mouse . Read more (PubMed: 24618541) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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