製品の概要
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製品名Nb2 nuclear extract lysate (prolactin stimulated)
Nb2 ライゼート 製品一覧 -
製品の詳細Nb2 nuclear extract lysate
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特記事項The Nb2 nuclear extract derived from rat lymphocytes have been collected in Lysis Buffer after a 20 minute stimulation with 25 ng/ml of prolactin at 37°C immediately prior to harvesting. The protein content has been determined by a Bradford-based assay.
製品の特性
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製品の状態Liquid
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保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9 -
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研究分野
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背景The Nb-2 rat lymphoma line was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Cells have a modified prolactin receptor and are used for in vitro bioassay of lactogenic hormones, studies of the mechanisms of lactogen induced mitogenesis, studies into the action of vinca alkaloids and studies of tumour progression.
参考文献
ab14869 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"