製品の概要

  • 製品名Anti-Nav1.8 antibody [S134-12]
    Nav1.8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S134-12] to Nav1.8
  • 特異性No cross reactivity against other Nav channels.
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Monkey
  • 免疫原

    Fusion protein, corresponding to amino acids 1724-1956 of Rat Nav1.8 (UniProt Q62968).

  • ポジティブ・コントロール
    • COS cell lysate transiently expressing Nav1.8

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 220 kDa.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.
  • 組織特異性Expressed in the dorsal root ganglia and sciatic nerve.
  • 関連疾患Episodic pain syndrome, familial, 2
  • 配列類似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.
    Contains 1 IQ domain.
  • ドメインThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻訳後修飾Ubiquitinated by NEDD4L; which promotes its endocytosis.
    Phosphorylation at Ser-1451 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • 細胞内局在Cell membrane. It can be translocated to the cell membrane through association with S100A10.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPN3 antibody
    • mPN3 antibody
    • Peripheral nerve sodium channel 3 antibody
    • Pn3 (gene name) antibody
    • PN3 antibody
    • SCN10A antibody
    • SCNAA_HUMAN antibody
    • Sensory neuron sodium channel antibody
    • Sns (gene name) antibody
    • SNS antibody
    • Sodium channel protein type 10 subunit alpha antibody
    • Sodium channel protein type X alpha subunit antibody
    • Sodium channel protein type X subunit alpha antibody
    • Voltage-gated sodium channel alpha subunit Nav1.8 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.8 antibody
    see all

Anti-Nav1.8 antibody [S134-12] 画像

  • Overlay histogram showing SH-SY5Y cells stained with ab93616 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93616, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Nav1.8 antibody [S134-12] (ab93616) 使用論文

This product has been referenced in:
  • Cejudo-Roman A  et al. The voltage-gated sodium channel nav1.8 is expressed in human sperm. PLoS One 8:e76084 (2013). Flow Cyt, ICC/IF ; Human . Read more (PubMed: 24086692) »
  • Ramachandra R  et al. Identification of CaV channel types expressed in muscle afferent neurons. J Neurophysiol 110:1535-43 (2013). Read more (PubMed: 23843437) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
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投稿 Sep 02 2014

The specificity of these antibodies to their target Nav channel was determined by performing western blot analysis on lysates from various HEK cell lines expressing one Nav channel. These antibodies were used to detect the presence of the channel in th...

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Thank you for contacting us in regards to the anti-Nav1.8 antibodies ab66743, ab83936, ab93616. I am sorry for the delay in my reply, I have been seeking information in regards to the IHC protocols used with these antibodies.

The antibodies y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"