製品の概要
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製品名Anti-Nav1.6 antibody
Nav1.6 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Nav1.6
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由来種Rabbit
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アプリケーション適用あり: IHC-FrFl, ICC/IF, IHC-P, IHC-Fr, WB, IHC-FoFrmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide derived from N terminal of human Nav1.6.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: Whole serum -
精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab65166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FrFl | Use at an assay dependent concentration. See Abreview. | |
| ICC/IF | Use at an assay dependent concentration. See Abreview. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 220 kDa. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 27905510 |
ターゲット情報
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機能Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.
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組織特異性Isoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages.
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配列類似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.
Contains 1 IQ domain. -
ドメインThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
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翻訳後修飾May be ubiquitinated by NEDD4L; which would promote its endocytosis.
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細胞内局在Membrane and Cytoplasmic vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 6334 Human
- Entrez Gene: 20273 Mouse
- Entrez Gene: 29710 Rat
- Omim: 600702 Human
- SwissProt: Q9UQD0 Human
- SwissProt: Q9WTU3 Mouse
- SwissProt: O88420 Rat
- Unigene: 710638 Human
see all -
別名
- CerIII antibody
- CIAT antibody
- EIEE13 antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Nav1.6 antibody (ab65166)PFA perfusion-fixed sections of rat hippocampus (following 21 fdays of KA treatment) using ab65166 at 1/600 dilution (red). Co-localization with Nestin (green) is shown.
Data taken from PMID 27905510 (Figure 7E).
Remarkable alterations of Nav1.6in reactive astrogliosis during epileptogenesis
HongyanZhu, Yuxiao Zhao*, HaoWu, Nan Jiang, ZiyWang, Weide Lin, Jiahui Jin & Yonghua JiScientific Reports | 6:38108 | DOI: 10.1038/srep38108
This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
プロトコール
参考文献
This product has been referenced in:
- Zhu H et al. Remarkable alterations of Nav1.6 in reactive astrogliosis during epileptogenesis. Sci Rep 6:38108 (2016). IHC-P ; Rat . Read more (PubMed: 27905510) »
- Wen M et al. Upregulation of RBFOX1 in the malformed cortex of patients with intractable epilepsy and in cultured rat neurons. Int J Mol Med 35:597-606 (2015). IHC-P . Read more (PubMed: 25571999) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"