Customer reviews and FAQs

製品の概要

  • 製品名Anti-Nav1.6 antibody
    Nav1.6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nav1.6
  • アプリケーション適用あり: IHC-FrFl, ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from N terminal of human Nav1.6.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl Use at an assay dependent concentration. See Abreview.
ICC/IF Use at an assay dependent concentration. See Abreview.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 220 kDa.

ターゲット情報

  • 機能Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. In macrophages and melanoma cells, isoform 5 may participate in the control of podosome and invadopodia formation.
  • 組織特異性Isoform 5 is expressed in non-neuronal tissues, such as monocytes/macrophages.
  • 配列類似性Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.
    Contains 1 IQ domain.
  • ドメインThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • 翻訳後修飾May be ubiquitinated by NEDD4L; which would promote its endocytosis.
  • 細胞内局在Membrane and Cytoplasmic vesicle. Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • CerIII antibody
    • CIAT antibody
    • EIEE13 antibody
    • hNa6/Scn8a voltage gated sodium channel antibody
    • MED antibody
    • Motor endplate disease antibody
    • NaCh 6 antibody
    • NaCh6 antibody
    • Nav 1.6 antibody
    • Nbna1 antibody
    • peripheral nerve protein type 4 antibody
    • PN 4 antibody
    • PN4 antibody
    • SCN8A antibody
    • SCN8A_HUMAN antibody
    • Sodium channel protein type 8 alpha subunit antibody
    • Sodium channel protein type 8 subunit alpha antibody
    • Sodium channel protein type VIII alpha subunit antibody
    • Sodium channel protein type VIII subunit alpha antibody
    • Sodium channel voltage gated type VIII alpha antibody
    • Sodium channel voltage gated type VIII alpha polypeptide antibody
    • Sodium channel voltage gated type VIII alpha subunit antibody
    • Voltage gated sodium channel subunit alpha Nav1.6 antibody
    • Voltage-gated sodium channel subunit alpha Nav1.6 antibody
    see all

Anti-Nav1.6 antibody (ab65166) 使用論文

This product has been referenced in:
  • Zhu H  et al. Remarkable alterations of Nav1.6 in reactive astrogliosis during epileptogenesis. Sci Rep 6:38108 (2016). IHC-P ; Rat . Read more (PubMed: 27905510) »
  • Wen M  et al. Upregulation of RBFOX1 in the malformed cortex of patients with intractable epilepsy and in cultured rat neurons. Int J Mol Med 35:597-606 (2015). IHC-P . Read more (PubMed: 25571999) »

See all 4 Publications for this product

ab65166 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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ab65166 の abreview を投稿する ab65166 に関する問い合わせをする

12 Abreviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nav1.6 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Human Tissue sections (fetal spinal cord nerve fibers)
Specification fetal spinal cord nerve fibers
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
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Username

Mr. Heiko Locher

Verified customer

投稿 Sep 23 2013

Thanks for your help however I believe that I will try another company. Can you please arrange a refund for this purchase?

Thanks,

Thank you very much for your reply, and I am very sorry for all of the trouble with these antibodies.

Your credit note ID is ***

If payment has already been made on the original order and you wish to receive a refund, please ask your...

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1) Are you still using rat hippocampus lysates? How much protein was loaded per lane of the gel?
Yes , I am and loaded 30ug per lane.

2) Could you describe the electrophoresis and gel conditions (% of the gel etc.)?
I used a 6% tri...

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Thank you for sending the additional information and for your patience.

I forwarded your protocol details to the lab for a second look, and we would have expected the antibody to work in the conditions that you've described. I am very sorry t...

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I tired antibody (ab65166), however again I did not have a band present. Is there anything else I can try?

Thanks,

Thank you very much for keeping me updated about the results with ab65166.

I'm sorry to hear that there was still no band on the blot. Would you mind telling me some more about the protocol you used with ab65166?

1) Are you still us...

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Ab83764 did not work in Western blot with rat lysate. Would like to try ab65166 instead.

Thank you very much for your calls today and for letting us know about the trouble with ab83764 in Western blot.

Please keep me updated about the results after using ab65166 with your samples.

I look forward to hearing from you. If ...

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Inquiry: Dear Sir/Madam, I recently bought an anti-Nav 1.6 antibody from abcam. However, it does not appear to be effective. I have used it to stain longitudinal frozen sections of spinal cord tissue (fixed using PFA) and the purpose of staining is to ...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for your helpful advice. I used Ponceau S on my most
recent blot and was able to visualize transfer of both my MW marker
and proteins in my cell lysate. It's certainly possible, however,
I could be getting over-transfe...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your suggestions. In reviewing my previous blots, I
realized that all the ones I had run with the commercial cell lysate
had been stained with Amido Black, which made them unsuitable for
stripping and re-probing. I conseque...

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Thank you for your reply.


I have a few additional suggestions. I am not sure if my colleague had mentioned those to you:

1) You can check the transfer with Ponceau S (which is a reversible membrane stain) to be sure that you tr...

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No signal in western blots

Thank you for calling and bringing this issue to our attention.

I just wanted to make sure you were loading the correct amount of protein and to make another suggestion regarding the blocking step.

For the brain lysate, ab30151, y...

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Western blot abreview for Anti-Nav1.6 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole brain homogenate)
Loading amount 10 µg
Specification Whole brain homogenate
Gel Running Conditions Reduced Denaturing (Bis-tris 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Mr. Brian Hitt

Verified customer

投稿 May 11 2010

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"