Natural Streptolysin O (Hemolytic streptococcus) protein (ab63978)

製品の概要

  • 製品名Natural Streptolysin O (Hemolytic streptococcus) protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Natural
  • 由来Escherichia coli
  • アミノ酸配列
    • 配列MGGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSR NKQN TANTETTTTN EQPKPESSEL TTEKAGQKMD DMLNSNDMIK LAPKEMPLES AEKEEKKSED NKKSEEDHTE EINDKIYSLN YNELEVLAKN GETIENFVPK EGVKKADKFI VIERKKKNIN TTPVDISIID SVTDRTYPAA LQLANKGFTE NKPDAVVTKR NPQKIHIDLP GMGDKATVEV NDPTYANVST AIDNLVNQWH DNYSGGNTLP ARTQYTESMV YSKSQIEAAL NVNSKILDGT LGIDFKSISK GEKKVMIAAY KQIFYTVSAN LPNNPADVFD KSVTLKELQR KGVSNEAPPL FVSNVAYGRT VFVKLETSSK SNDVEAAFSA ALKGTDVKTN GKYSDILENS SFTAVVLGGD AAEHNKVVTK DFDVIRNVIK DNATFSRKNP AYPISYTSVF LKNNKIAGVN NRSEYVETTS TEYTSGKINL SHQGAYVAQY EILWDEINYD DKGKEVITKR RWDNNWYSKT SPFSTVIPLG ANSRNIRIMA RECTGLAWEW WRKVIDERDV KLSKEINVNI SGSTLSPYGS ITYK

特性

Our Abpromise guarantee covers the use of ab63978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

     Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37? for 30 minutes.

     

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    Purity >98% SDS-PAGE.
  • 製品の状態Liquid
  • 備考

    Information about health hazards:

    Target: Cholesterol on human and animal cell membrane

    Health Hazards: May be fatal if enters bloodstream.

    LD50-Lethal dose (50 percent kill) intravenous,

    Rabbit, 1500 ng/kg (Ref :PHTHDT Pharmacology and Therapeutics. (Pergamon Press Ltd.,Headington Hill Hall, Oxford OX3 0BW, UK) Vol.(Issue) 11, Page 661,1981 )

    Guinea pig, 12 ug/kg (Ref: BICMBE Biochimie. (SPPIF, B.P.22, F-41353 Vineuil, France, Vol.(Issue)55,Page 1187, 1973)

    Toxicity is much less when introduced via other routes of entry like interdermal injection.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, PBS, 1mM DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Streptolysin O
    • Streptolysin O (Thiol-activated cytolysin) slo
  • 関連性Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.

Natural Streptolysin O (Hemolytic streptococcus) protein 画像

  • ab63978 on SDS-PAGE.

Natural Streptolysin O (Hemolytic streptococcus) protein (ab63978) 使用論文

This product has been referenced in:
  • Lee YK  et al. Modeling of Friedreich ataxia-related iron overloading cardiomyopathy using patient-specific-induced pluripotent stem cells. Pflugers Arch 466:1831-44 (2014). Functional Studies . Read more (PubMed: 24327207) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

I am afraid I am not aware of any publication regarding the inactivation method of Streptolysin O.

However, it can be done by dialyzing against 0.4% formaldehyde in 100 mM at 30℃ for 3 days.

I ...

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Streptolysin O (SLO), prototype of the cholesterol-binding family of bacterial exotoxins, forms very large pores in the plasma membrane of mammalian cells (http://www.ncbi.nlm.nih.gov/pubmed/8593102). I am afraid injec...

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Thank you for contacting Abcam.

Our streptolysin O (SLO) is active without pretreatment probably because the solution contains 1 mM DTT. We measured pore-forming activity by hemolysin activity.

If there is anything else I can help y...

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Thank you for contacting Abcam.
To find the extinction coefficient, I used the follwing link:
http://encorbio.com/protocols/Prot-MW-Abs.htm
For Streptolysin O protein ;
There are 571 Amino acids and Molecular Weight is 63.64 KiloDal...

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Thank you for contacting us.

The activity of this Streptolysin O proteinis measured by applying the protein to 3% sheep red blood cells in PBS, pH 7.4 at 37C for 30 minutes and counting the lysed cells under the microscope. Using this protoco...

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This protein is not produced under GMP conditions. It is not for human use and is for research use only. I hope this is helpful. Please contact me again if you need any further information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"