製品の概要

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • アクセッション番号P00761
    • 生物種Pig
    • 配列FPTDDDDKIV GGYTCAANSI PYQVSLNSGS HFCGGSLINS QWVVSAAHCY KSRIQVRLGE HNIDVLEGNE QFINAAKIIT HPNFNGNTLD NDIMLIKLSS PATLNSRVAT VSLPRSCAAA GTECLISGWG NTKSSGSSYP SLLQCLKAPV LSDSSCKSSY PGQITGNMIC VGFLEGGKDS CQGDSGGPVV CNGQLQGIVS WGYGCAQKNK PGVYTKVCNY VNWIQQTIAA N
    • 分子量24 kDa
    • 領域1 to 231

特性

Our Abpromise guarantee covers the use of ab101090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性 Approximately 5000 Units/mg material.
    The spectrophotometric assay to determine the activity of trypsin measures the increase in absorbance at 253 nm due to N-a-benzoyl arginine as it is cleaved from N-a-benzoyl arginine ethyl ester, with the concomitant formation of ethanol.
  • 製品の状態Lyophilised
  • 備考


    Trypsin catalyzes the cleavage of peptide bonds in proteins at the carboxyl side of arginine and lysine residues, except when either is followed by a proline residue. Trypsin is commonly used to remove adherent cells during cell culture manipulations carried out in vitro.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20ºC.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成This product should ideally be re-desiccated under vacuum over silica gel. Dissolves readily at 1mg/mL in 1 mM Hydrochloric acid, can be used in 67mM potassium phosphate buffer to assay for trypsin activity

関連情報

  • 別名
    • Alpha-trypsin chain 2
    • Anionic trypsinogen
    • Beta trypsin
    • Beta-trypsin
    • Brain trypsinogen
    • Cationic trypsinogen
    • Digestive zymogen
    • Mesotrypsin
    • Mesotrypsinogen
    • MGC111183
    • MGC120174
    • MGC120175
    • MGC149362
    • MTG
    • Nonfunctional trypsin 1
    • Pancreatic trypsinogen III
    • Protease Serine 1
    • Protease serine 1 (trypsin 1)
    • Protease serine 2
    • Protease serine 2 (trypsin 2)
    • Protease serine 2 preproprotein
    • Protease serine 3
    • Protease serine 3 (mesotrypsin)
    • Protease serine 4 (trypsin 4 brain)
    • Prss1
    • PRSS2
    • PRSS3
    • PRSS4
    • Serine protease 1
    • Serine protease 2
    • Serine protease 3
    • Serine protease 4
    • T9
    • TRP1
    • TRY1
    • TRY1_HUMAN
    • TRY2
    • TRY3
    • TRY4
    • TRY8
    • TRYP1
    • TRYP2
    • Trypsin 1
    • Trypsin 2
    • Trypsin 3
    • Trypsin I
    • Trypsin II
    • Trypsin III
    • Trypsin IV
    • Trypsinogen 1
    • Trypsinogen 2
    • Trypsinogen 4
    • Trypsinogen 5
    • Trypsinogen A
    • Trypsinogen, anionic
    • Trypsinogen, cationic
    • Ttrypsinogen IV
    see all
  • 機能Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • 関連疾患Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt

Natural pig Trypsin protein (ab101090) 使用論文

ab101090 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101090.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"