Natural Human Urokinase protein (FITC) (ab93152)

製品の概要

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • 生物種Human
  • 標識FITC. Ex: 493nm, Em: 528nm

特性

Our Abpromise guarantee covers the use of ab93152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.
    > 98% pure by SDS PAGE
  • 製品の状態Liquid
  • 備考Solubility: > 2 mg/mL Protect from light
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M Sodium acetate, 1mM EDTA, pH 5.0

関連情報

  • 別名
    • ATF
    • ATF uPA
    • BDPLT5
    • Plasminogen activator
    • Plasminogen activator urinary
    • Plasminogen activator urokinase
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    • Urokinase type plasminogen activator precursor
    • Urokinase-type plasminogen activator chain B
    see all
  • 機能Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • 組織特異性Expressed in the prostate gland and prostate cancers.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 細胞内局在Secreted.
  • Information by UniProt

Natural Human Urokinase protein (FITC) 画像

  • 10% SDS-PAGE
    Lane 1: ab93152 (2 µg), non reduced
    Lane 2: ab93152 (2 µg) plus Human PAI1 (stable mutant form) (10 µg), non reduced
  • 10% SDS-PAGE
    Lane 1: ab93152 (2 µg), non reduced
    Lane 2: ab93152 (2 µg) plus Human PAI1 (stable mutant form) (10 µg), non reduced
    Lane 3: Prestained standard

Natural Human Urokinase protein (FITC) (ab93152) 使用論文

ab93152 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: Prior to using for fluoresecent confocal microscopy ab93152 (FITC-uPA) was tested for fibrinolytic activity by measuring clot lysis using a turbidity assay. Clot lysis was observed with 100 pM ab93152 (50 % lysis time = 130 min) prior to aliquoting and freezing as instructed in the data sheet. However, after freeze thawing activity was reduced (50 % lysis time = 248 min)
For observation of lysis by fluorescent confocal microscopy 75 nM ab93152 was used. No signal for the FITC uPA could be detected.
Sample: Human Recombinant protein

Primary antibody (in addition to 'Urokinase protein (FITC) (Human)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
Abcam response: Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
Username

Dr. Claire Whyte

Verified customer

投稿 Nov 27 2012

Thank you very much for your interest in our products.
As we haven't tested this ourself, it is hard to predict if these proteins will work in a functional study. Therefore, I can offer a discount off a future purchase if you buy ab92679 and ab931...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"