Native human Thrombin protein (Active) (ab62452)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Native human Thrombin protein (Active)
Thrombin タンパク質・ペプチド 製品一覧 -
生理活性
This product can be used for cleavage of fusion proteins. The activity varies form lot to lot. It is typically 3800 U/mg.
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精製度
> 95 % SDS-PAGE. -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab62452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Concentration varies from lot to lot but is between 5mg/ml and 10mg/ml.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituent: 50% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all -
機能
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
細胞内局在
Secreted, extracellular space. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab62452 は 3 報の論文で使用されています。
- Schmidt C et al. A multiparametric fluorescence assay for screening aptamer-protein interactions based on microbeads. Sci Rep 12:2961 (2022). PubMed: 35194086
- Amick KA et al. Plasma glycocholic acid and linoleic acid identified as potential mediators of mitochondrial bioenergetics in Alzheimer's dementia. Front Aging Neurosci 14:954090 (2022). PubMed: 36212044
- Park HJ et al. One-pot colorimetric detection of molecules based on proximity proteolysis reaction. Biosens Bioelectron 188:113349 (2021). PubMed: 34030090