Native human Prethrombin 2 protein (ab62535)
Key features and details
- Expression system: Native
- Purity: > 95% Ion Exchange Chromatography
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Native human Prethrombin 2 protein -
生理活性
Activity was measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin. -
精製度
> 95 % Ion Exchange Chromatography.
Prepared from fresh frozen human plasma. Purity was determined by SDS-PAGE analysis. -
発現系
Native -
タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human
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特性
Our Abpromise guarantee covers the use of ab62535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol, 50% Water
50% H2OThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Prothrombin
- Prothrombin precursor
- PT
- Serine protease
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関連性
Prethrombin 2, the smallest single-chain immediate precursor of a-thrombin (corresponding to residues Thr272 to Glu579 in prothrombin), has one glycosylation site at Asn373 and four disulfide bonds, Cys293-Cys439, Cys348-Cys364, Cys493-Cys507, and Cys521-Cys551. alpha-Thrombin can be derived from prothrombin by two pathways, each of which generates different intermediate products. In one pathway, human prothrombin is converted relatively slowly to alpha-thrombin in the presence of factor Xa and Ca2+ ions by initial cleavage at residue (Arg) 271/(Thr) 272 with consequent production of fragment 1.2 and prethrombin 2. Prethrombin 2 is then cleaved at R 320 with consequent generation of alpha-thrombin. In the other pathway, initial cleavage at R 320 yields an intermediate product, meizothrombin. -
細胞内局在
Secreted, extra cellular space
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab62535 は 1 報の論文で使用されています。
- Jonebring A et al. Expression and characterization of recombinant ecarin. Protein J 31:353-8 (2012). PubMed: 22528138