Native human Plasmin protein (Active) (ab90928)
Key features and details
- Expression system: Native
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Native human Plasmin protein (Active)
Plasmin タンパク質・ペプチド 製品一覧 -
生理活性
15 units/mg protein
One unit is defined as the amount of enzyme that hydrolyzes one µmole of tosyl-Gly-Pro-Lys-pNA per minute at 25°C, pH 7.8. Note: One unit = 1.25 CU.
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精製度
>= 95 % SDS-PAGE. -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
予測される分子量
81 kDa
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab90928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Extinction Coeffcient (E) 0.1% at 280nm 1cm pathway =1.71
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20ºC.
Information available upon request.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成We recommend that this product be resuspended in de-ionized H2O to the original volume.
関連情報
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別名
- Plasmin light chain B
- Plasminogen
- PLG
- PLMN_HUMAN
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機能
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
組織特異性
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
関連疾患
Plasminogen deficiency -
配列類似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
ドメイン
Kringle domains mediate interaction with CSPG4. -
翻訳後修飾
N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
細胞内局在
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
画像
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4-12% Bis-Tris NuPAGE gel.
Lane 1: Plasmin - 5 μg (reduced/ heated)
Lane 2: Plasmin - 10 μg (reduced/ heated)
Lane 3: Plasmin - 20 μg (reduced/ heated)
Lane 4: LMW Standard
Lane 5: Plasmin - 5 μg (non-reduced/ no heat)
Lane 6: Plasmin - 10 μg (non-reduced/ no heat)
Lane 7: Plasmin - 20 μg (non-reduced/ no heat)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab90928 は 2 報の論文で使用されています。
- Strittmatter T et al. Programmable DARPin-based receptors for the detection of thrombotic markers. Nat Chem Biol 18:1125-1134 (2022). PubMed: 35941237
- Nishimura M et al. Extracellular cleavage of collagen XVII is essential for correct cutaneous basement membrane formation. Hum Mol Genet 25:328-39 (2016). PubMed: 26604146