Natural Human LRG1 protein (ab115506)

製品の概要

製品の詳細

  • 由来
    Native
  • 由来
    Native
  • アミノ酸配列
    • アクセッション番号
    • 生物種
      Human
    • 分子量
      34 kDa
    • 領域
      36 to 347

特性

Our Abpromise guarantee covers the use of ab115506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    ELISA

    Functional Studies

  • エンドトキシン・レベル
    < 1.000 Eu/µg
  • 製品の状態
    Lyophilised
  • 備考
    All blood samples used for protein preparation were tested and found negative for HBsAg, anti-HCV, HIV Ag/Ab, and syphilis.
    Identification of LRG from human serum by LC-MS/MS – The band of related protein was excised from the polyacrylamide gel and in-gel digested by trypsin (20ng/µL) for 2 hours in 40°C. Tryptic peptides were analysed by LC-MS/MS. The MASCOT score for LRG was 569 with 28% sequence coverage. MS/MS analysis of seven peptides yielded significant individual ions scores (for scores >50, p<0.05)
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C.

    pH: 7.50
    Constituents: 0.12% Sodium chloride, 0.24% Tris

  • 再構成
    Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

関連情報

  • 別名
    • 1300008B03Rik
    • 2310031E04Rik
    • A2GL
    • A2GL_HUMAN
    • HMFT1766
    • Leucine rich alpha 2 glycoprotein
    • Leucine-rich alpha-2-glycoprotein
    • Leucine-rich alpha-2-glycoprotein 1
    • Leucine-rich alpha-2-glycoprotein precursor
    • LRG
    • LRG1
    see all
  • 組織特異性
    Plasma.
  • 配列類似性
    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt

画像

  • SDS-PAGE analysis of Human LRG1: 12% gel stained with Coomassie Brillant Blue R250
    Lane 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

参考文献

ab115506 has not yet been referenced specifically in any publications.

レビューと Q&A

The laboratory reports that in vitro affinity studies with LRG and Cytochrome C confirmed that the LRG protein specifically binds to human Cytochrome c1, heme protein.

The protein is isolated from a pooled serum, so is assumed to be glycosyl...

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Native Leucine-Rich Alpha-2-Glycoprotein (ab115506) is isolated from human pooled serum by cascade of appropriate chromatographic techniques like IEC, affinity chromatography, GPC. Native state of the protein has been maintained during whole process of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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