Natural Human IgG protein (ab98981)



  • 由来
  • 由来
  • アミノ酸配列
    • アクセッション番号


Our Abpromise guarantee covers the use of ab98981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Radial Immunodiffusion


  • 精製度
    96 % .
    IgA and IgM levels are <1% of the total IgG content by RID. ab98981 shows a single band corresponding to gamma electrophoretic mobility by Cellulose Acetate Electrophoresis.
  • 製品の状態
  • 備考

    Starting material serum donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.

    IgG from pooled normal human serum/plasma

  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

    Preservative: None
    Constituents: 0.02M Ammonium bicarbonate
    (May contain traces of buffer salts)

  • 再構成
    Reconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.


  • 別名
    • FLJ39988
    • FLJ40036
    • FLJ40253
    • FLJ40587
    • FLJ40789
    • FLJ40834
    • G1m marker
    • G2m marker
    • G3m marker
    • G4m marker
    • HDC
    • Heavy chain disease protein
    • Human immunglobulin G
    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG heavy chain locus
    • IGHG 1
    • IGHG 2
    • IGHG 3
    • IGHG 4
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobulin Gm1
    • Immunoglobulin Gm3
    • Immunoglobulin Gm4
    • Immunoglobulin heavy constant gamma 1
    • immunoglobulin heavy constant gamma 1 (G1m marker)
    • Immunoglobulin heavy constant gamma 2
    • immunoglobulin heavy constant gamma 2 (G2m marker)
    • Immunoglobulin heavy constant gamma 3
    • immunoglobulin heavy constant gamma 3 (G3m marker)
    • Immunoglobulin heavy constant gamma 4
    • immunoglobulin heavy constant gamma 4 (G4m marker)
    • MGC117419
    • MGC45809
    see all
  • 関連性
    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • 細胞内局在


  • The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.


ab98981 has not yet been referenced specifically in any publications.

レビューと Q&A

These two IgG proteins are similar in many ways including origin, and levels of purity. However they do differ in regards to the storage buffer in which they are provided. ab98981: Preservative: None Constituents: 0.02M Ammonium bicarbonate (May co...

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I would recommend use of a secondary antibody against mouse IgG which has been pre-absorbed against human IgG. These antibodies are run through columns containing IgGs of many species and and antibody which reacts with those IgGs will be trapped in the...

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Thank you for contacting us and I am sorry for the delay in getting back to you.

I cannot see why the Human IgG protein (ab98981) could not be used in cell culture. However, I would bear in mind that the product has not been sterilized and may...

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Thank you for your enquiry and your interest.

These two products are from different sources and there are slight variations between them.

ab91102 is from one patient, tested in SDS-PAGE and the storage buffer contains 0.05% Sodium A...

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Thank you for your patience in this matter.

The storage at 2-8 is relevant to the freeze dried product. Once resuspended we would not really recommend storage at 2-8 for no more than 1-2 weeks, and this would depend on the diluent used. We wou...

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Thank you and your customer for your inquiry.

Unfortunately, the Kd (Dissociation constant) of ab7500 was not experimentally tested and we cannot provide any data for this.

But I can confirm that ab7500 is guaranteed for peptide ELI...

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Thank you for contacting us.

This product has not been used in ELISA and as such is not covered by our Abpromise guarantee in this assay. However, after reviewing your idea, I see no reason to believe that this product (ab91102) or our other ...

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Thank you for your enquiry. The denaturing process is reversible in some cases. The original structure of some proteins can be regenerated by removal of the denaturing agent. I have attached a link to Wikipedia regarding protein denaturing if this ...

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I have contacted our lab on your behalf regarding the issues you are having with the IgG protein you bought from Abcam. Our lab personnel are requesting the lot number of the product you have so that they can test it and see if what else is present tha...

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