Native human Factor V protein (ab62414)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Native human Factor V protein
Factor V タンパク質・ペプチド 製品一覧 -
生理活性
Lot dependant, Determined by clotting assay.
-
精製度
> 95 % SDS-PAGE.
Human Factor V is prepared from fresh frozen human plasma using immunoaffinity chromatography. -
発現系
Native -
タンパク質長
Full length protein -
Animal free
No -
由来
Native -
-
生物種
Human -
実際の分子量
330 kDa
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab62414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Water, 50% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Activated protein C cofactor
- APC cofactor
- Coagulation factor V
see all -
機能
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. -
組織特異性
Plasma. -
関連疾患
Factor V deficiency
Thrombophilia due to activated protein C resistance
Budd-Chiari syndrome
Ischemic stroke
Pregnancy loss, recurrent, 1 -
配列類似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains. -
ドメイン
Domain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats. -
翻訳後修飾
Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
Activated protein C inactivates factor V and factor Va by proteolytic degradation.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted. - Information by UniProt
画像
-
Gel: Novex 4-12% Bis-Tris
Load: 1 µg per lane
Buffer is MOPS
Standard: SeeBluePlus 2; Myosin (191 kDa), Phosphorylase B (97 kDa), BSA (64 kDa), Glutamic Dehydrogenase (51 kDa), Alcohol Dehydrogenase (39 kDa), Carbonic Anhydrase (28 kDa), Myoglobin Red (19 kDa), Lysozyme (14 kDa)
Note: Factor V is a very labile protein. Some degraded fragments are usually noticeable in preparations.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab62414 は論文での使用が確認できていません。