The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activity: Greater than 200 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Z-Arg-Arg-beta-naphthylamide per minute at 40°C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
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Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Yoon JY et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J3:e153 (2013).
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