Natural human Catalase protein (ab91026)

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製品の概要

製品の詳細

  • 由来
    Native
  • 由来
    Native
  • アミノ酸配列
    • 生物種
      Human

特性

Our Abpromise guarantee covers the use of ab91026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.

  • 製品の状態
    Liquid
  • 備考
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.39. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • 機能
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性
    Belongs to the catalase family.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt

画像

  • SDS-PAGE - Catalase protein (Active) (ab91026)
    SDS-PAGE - Catalase protein (Active) (ab91026)
    SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg Catalase (reduced/heated)
    Lane 2: 10 µg Catalase (reduced/heated)
    Lane 3: 20 µg Catalase (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg Catalase (non-reduced/no heat)
    Lane 6: 10 µg Catalase (non-reduced/no heat)
    Lane 7: 20 µg Catalase (non-reduced/no heat)

参考文献

ab91026 has not yet been referenced specifically in any publications.

ab91026 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

10
Votes: Highest First

Wwe received the replacement vial of ab91026 today. It was still frozen on dry ice when we received it, but we observed the same green substance and brown threads in the frozen vial. This was also a different lot of the protein: CT 201001. Can we get a...

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Thank you for contacting Abcam.

Having talked to the lab, they told me that frozen Catalase has the appearancethat you described. Therefore the vial is not contaminated as I first thought andso you can continue your experiments as normal. Read More

Mitosciences received a vial of ab91026 Catalase Protein Active Lot# ***** on January 4th, 2012 (Abcam order# *****). This vial was stored at -20C until yesterday, January 19, 2012, when contamination was observed in the frozen contents. There is green...

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Thank you for contacting Abcam.

I am sorry that the vial of ab91026 was contaminated upon arrival. I have set the product to ship on Monday and it should arrive to you on Tuesday morning. Your new order number is ******.

Please let ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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