Natural human Catalase protein (ab91026)

製品の概要

製品の詳細

  • 由来
    Native
  • 由来
    Native
  • アミノ酸配列
    • 生物種
      Human

特性

Our Abpromise guarantee covers the use of ab91026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性
    Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度
    > 95 % SDS-PAGE.

  • 製品の状態
    Liquid
  • 備考
    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.39. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Tris HCl, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • 機能
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • 関連疾患
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • 配列類似性
    Belongs to the catalase family.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt

画像

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg Catalase (reduced/heated)
    Lane 2: 10 µg Catalase (reduced/heated)
    Lane 3: 20 µg Catalase (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg Catalase (non-reduced/no heat)
    Lane 6: 10 µg Catalase (non-reduced/no heat)
    Lane 7: 20 µg Catalase (non-reduced/no heat)

参考文献

ab91026 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam.

Having talked to the lab, they told me that frozen Catalase has the appearancethat you described. Therefore the vial is not contaminated as I first thought andso you can continue your experiments as normal. Read More

Thank you for contacting Abcam.

I am sorry that the vial of ab91026 was contaminated upon arrival. I have set the product to ship on Monday and it should arrive to you on Tuesday morning. Your new order number is ******.

Please let ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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