Native human Catalase protein (ab91026)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Native human Catalase protein
Catalase タンパク質・ペプチド 製品一覧 -
生理活性
Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0 -
精製度
> 95 % SDS-PAGE. -
発現系
Native -
タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Human -
予測される分子量
230 kDa -
配列の追加情報
Purified from Human Erythrocytes. Four identical subunits each with a molecular weight of 58,000.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab91026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituent: 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Cas1
- CAT
- CATA_HUMAN
see all -
機能
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
関連疾患
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
配列類似性
Belongs to the catalase family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Peroxisome. - Information by UniProt
画像
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SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg Catalase (reduced/heated)
Lane 2: 10 μg Catalase (reduced/heated)
Lane 3: 20 μg Catalase (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 μg Catalase (non-reduced/no heat)
Lane 6: 10 μg Catalase (non-reduced/no heat)
Lane 7: 20 μg Catalase (non-reduced/no heat) -
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :Native human Catalase protein (ab91026) at 0.1 µg
Lane 2 :Native human Catalase protein (ab91026) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab91026 は 1 報の論文で使用されています。
- Czechowska E et al. Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption. Colloids Surf B Biointerfaces N/A:N/A (2018). PubMed: 30054048