Natural human Calpain 1 protein (ab91019)

製品の概要

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • 生物種Human

特性

Our Abpromise guarantee covers the use of ab91019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性

    Activity: Greater than or equal to 1,000 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one picomole of Suc-LLVY-AMC per minute at 25&degC.

  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Liquid
  • 備考Prepared from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 20mM Imidazole
    Constituents: 30% Glycerol, 5mM Beta mercaptoethanol, 1mM EDTA, 1mM EGTA, pH 6.8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名
    • Ca2 activated neutral protease
    • Calcium activated neutral proteinase
    • Calcium activated neutral proteinase small subunit
    • Calcium dependent protease small subunit
    • Calcium dependent protease small subunit 1
    • Calcium-activated neutral proteinase 1
    • Calpain 1
    • Calpain 1 large subunit
    • Calpain 1, (mu/I) large subunit
    • Calpain mu type
    • Calpain mu-type
    • Calpain regulatory subunit
    • Calpain small subunit 1
    • Calpain, large polypeptide L1
    • Calpain-1 catalytic subunit
    • Calpain-1 large subunit
    • CAN1_HUMAN
    • CANP
    • CANP 1
    • CANP small subunit
    • CANP1
    • CANPL 1
    • CANPL1
    • CAPN 1
    • CAPN1
    • Cell proliferation inducing protein 30
    • Cell proliferation-inducing gene 30 protein
    • Micromolar Calpain
    • Micromolar-calpain
    • Mu Calpain
    • muCANP
    • muCL
    see all
  • 機能Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • 細胞内局在Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt

Natural human Calpain 1 protein 画像

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    1. 5 µg Calpain 1 (reduced /heated)
    2. 10 µg Calpain 1 (reduced/ heated)
    3. Molecular weight markers
    4. 5 µg Calpain 1 (non-reduced/no heat)
    5. 10 µg Calpain 1 (non-reduced/no heat)
  • Anti-Calpain 1 antibody (ab2543) at 1 µg/ml + Natural human Calpain 1 protein (ab91019) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Natural human Calpain 1 protein (ab91019) 使用論文

ab91019 has not yet been referenced specifically in any publications.

Product Wall

The specific activity for GR23778 is 2126 Units/mg.

Hello. No, this is not exactly the same, the protein ab91019 comes from another source. They are very similar though. Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"