Natural E. coli beta Galactosidase protein (ab78141)

製品の概要

製品の詳細

  • 由来Natural
  • 由来Escherichia coli
  • アミノ酸配列
    • 生物種Escherichia coli
    • 分子量114 kDa
    • 領域2 to 1024

特性

Our Abpromise guarantee covers the use of ab78141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生理活性Does not react with human serum.
  • アプリケーション

    SDS-PAGE

  • 精製度> 95 % SDS-PAGE.

  • 製品の状態Liquid
  • 備考Does not react with human serum.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8

関連情報

  • 別名
    • Beta D galactosidase
    • Beta gal
    • Beta galactosidase
    • ECK0341
    • JW0335
    • Lactase
    • lacZ
    see all
  • 関連性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 細胞内局在Cytoplasmic

Natural E. coli beta Galactosidase protein (ab78141) 使用論文

This product has been referenced in:
  • Ruggiero L  et al. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Proc Natl Acad Sci U S A 109:8145-8 (2012). Read more (PubMed: 22566632) »

See 1 Publication for this product

Product Wall

Application Other

Review text: used as control protein during ex vivo interaction assay. worked well, no interaction with isolated live tissues, as expected.
Sample: Mouse Tissue lysate - whole

Primary antibody (in addition to 'beta Galactosidase protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
PubMed ID: 22566632: View
Username

Abcam user community

Verified customer

投稿 Aug 13 2012

Thank you for contacting Abcam.

Attached, please find the CoC documents you requested for ab78141.


Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we...

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Thank you for your inquiry.
I investigated why one vial froze and the other did not.
I can now confirm that Lot #GR21906-1 and Lot #GR69274-2 both contain the same buffer. The 8M urea may or may not freeze at -20C storage. I would like to rea...

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Thank you for contacting abcam with this request. I am attaching a certificate of compliance for the product as you requested.  Please see the link below for an explanation of a CoC. http://www.abcam.com/index.html?pageconfig=technicalfaqs#19 As ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"