Natural Cow 160 kD Neurofilament Medium protein (ab73759)

製品の概要

  • 製品名Natural Cow 160 kD Neurofilament Medium protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • 生物種Cow
    • 分子量160 kDa
    • 配列の追加情報Source = bovine spinal cord

特性

Our Abpromise guarantee covers the use of ab73759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    ELISA

    Western blot

  • 精製度> 95 % SDS-PAGE.
    Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • 製品の状態Lyophilised
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 6M Urea, 2mM DTT, 10mM Sodium phosphate, 1mM EDTA, pH 7.5

  • 再構成Reconstitute in sterile 18MO-cm H2O. Upon reconstitution NRFab73759 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

関連情報

  • 別名
    • 150kDa medium
    • NEF3
    • NEFM
    • Neurofilament 3
    • NF160
    • NFM
    see all
  • 関連性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • 細胞内局在Cytoplasm

Natural Cow 160 kD Neurofilament Medium protein 画像

  • All lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilution

    Lane 1 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.1 µg
    Lane 2 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Natural Cow 160 kD Neurofilament Medium protein (ab73759) 使用論文

ab73759 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"