Natural Clostridium difficile Toxin B protein (ab124001)

製品の概要

  • 製品名Natural Clostridium difficile Toxin B protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • アクセッション番号P18177
    • 分子量270 kDa
    • 領域2 to 2366

特性

Our Abpromise guarantee covers the use of ab124001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 精製度> 95 % SDS-PAGE.
    Clostridium difficile Toxin B has been highly purified.
  • 製品の状態Lyophilised
  • 備考This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.

    Functional Studies Protocol

    1.1 Vero cells are plated out the night before on a 96-well plate at a concentration of 20K cells per well (in 100ml), then incubated at 37°C, 5% CO2. The following day they should be lightly confluent.

    1.2 Lyophilised toxin/toxoid is resuspended as per the instructions in the certificate of analysis, and diluted in duplicate to 0.1mg/ml in DMEM (200ml total) without FBS in the first well of a non-TC-treated 96-well plate. A serial 5-fold dilution is performed (40ul in 160ul) in the same media using a multichannel for 11 dilutions in total, leaving the 12th dilution as a media control.

    1.3 20ul of each dilution is then pipetted onto each well of the pre-prepared Vero cells and then incubated at 37°C, 5% CO2 for 24 hours.

    1.4 The following day, the cells are examined using an inverted phase contrast tissue culture microscope. Cells with active toxin on should be rounded, cells without significant toxicity should look like the control cells.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle.

    Constituents: 1.19% HEPES, 5% Sucrose, 0.88% Sodium chloride

  • 再構成Reconstitute in 250µl sterile distilled water.

関連情報

  • 別名
    • C. difficile Toxin B
    • Clostridium difficile Toxin B
    • Cytotoxin B
    • tcdB
    • toxB
    • Toxin B
    see all
  • 関連性Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.
  • 細胞内局在Secreted

Natural Clostridium difficile Toxin B protein 画像

  • SDS-PAGE showing ab124001 at 270 kDa.

Natural Clostridium difficile Toxin B protein (ab124001) 使用論文

ab124001 has not yet been referenced specifically in any publications.

Product Wall



These products were purified from Clostridium difficile strain VPI10463 (which produces toxinotype 0 toxins). The toxins are active, each batch is tested by verocytotoxicity assay and demonstrated to be toxic to cells down to at least 5 ng/ml...

Read More

Thank you for contacting us.

In response to your questions:

1)The toxin is guaranteed to be stable for at least 1 month after reconstitution, (we have data that shows 6 weeks is still fine).

2)The toxin can be frozen for l...

Read More

Thank you for contacting us again.

I am delighted to send you the protocol of the cell toxicity assay with Vero cells





1 Verocycotoxicity Assay
1.1 Vero cells are plated out the night before on a 96-well plate a...

Read More

I have contacted the lab regarding your question and here is their answer:

Yes, it is fully cytotoxic and has been quality controlled in a vero cell cytotoxicity assay.

Vielen Dank für Ihren Anruf.

Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostri...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"