The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab63835 was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns.
No toxic effect was observed in two mice injected with 5 ug of C3 enzyme, showing little contamination of lethal toxins in this preparation.
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
ab63835 can be used for studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro and for the study of ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
Its activity tested as tested and stimulation of human keratinocytes in culture was observed at 50 ng/ml.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Mono-ADP-ribosyltransferase C3 from Clostridium botulinum D phage (Clostridium botulinum D bacteriophage). ADP-ribosylates eukaryotic Rho and Rac proteins on an asparagine residue. It has a Mass of 27.8kDa.
SDS-PAGE - Clostridium botulinum Exoenzyme C3 full length protein (ab63835)
ab63835 on SDS-PAGE (CBB staining). Molecular Weight: ~27.8 kDa.
has not yet been referenced specifically in any publications.