製品の概要

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • 配列の追加情報purified from natural source, either algae or cyanobacteria

特性

Our Abpromise guarantee covers the use of ab123473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    FRET

    Flow Cytometry

    Immunocytochemistry/ Immunofluorescence

  • 製品の状態Liquid
  • 備考

    Before use, centrifuge ab123472 suspension at 10 000g for 10 min at 4°C. Discard the supernatant and resuspend the pellet into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis. Abs/Em = 651/662 nm.

    The extinction coefficient of CL-APC at 650nm is 70,000 cm-1M-1.

     

     

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    pH: 7.00
    Note: Ammonium sulfate is around 40% for protein precipitation

関連情報

  • 別名
    • apcB
    • Allophycocyanin alpha chain
    • Allophycocyanin beta chain
    • apcA
  • 関連性Allophycocyanin is a protein from the light-harvesting phycobiliprotein family, along with phycocyanin, phycoerythrin and phycoerythrocyanin. It is an accessory pigment to chlorophyll. All phycobiliproteins are water soluble and therefore cannot exist within the membrane like carotenoids, but aggregate to form clusters that adhere to the membrane called phycobilisomes. Allophycocyanin absorbs and emits red light (650 & 660 nm max, respectively), is readily found in Cyanobacteria and red algae, and is a useful immunoassay tool. APC is made up of alpha and beta subunits and is present as a trimer (alpha beta)3. The trimer is unstable and susceptible to dissociation at low concentrations. The monomer, alpha beta, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is shifted to 620 nm. The chemically crosslinked APC (CL-APC) trimer is much more stable than the native protein, but still retains comparable spectroscopic properties. The extinction coefficient of CL-APC at 650 nm is 700,000 cm-1M-1.

Natural Allophycocyanin protein (ab123473) 使用論文

ab123473 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"