Natural Adenovirus Type 5 hexon protein (ab123995)

製品の概要

  • 製品名Natural Adenovirus Type 5 hexon protein
  • タンパク質長Full length protein

製品の詳細

  • 由来Natural
  • 由来Native
  • アミノ酸配列
    • アクセッション番号P04133
    • 分子量108 kDa
    • 領域2 to 952

特性

Our Abpromise guarantee covers the use of ab123995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    ELISA

  • 精製度> 95 % SDS-PAGE.
    Purification involves multiple steps including sequential size exclusion and ion exchange chromatography.
  • 製品の状態Liquid
  • 備考Hexon protein naturally self assembles as part of the virus life cycle. When the protein is purified we also see evidence of self assembly such as rapid loss of protein upon serial dilution. The storage buffer and ELISA coating buffer play an important role in the likelihood of self assembly and thereby can affect results when using purified hexon as antigen in ELISAs and other such assays. Many common ELISA coating buffers increase self assembly in hexon protein as they are designed to increase efficiency of antigen adsorption. This results in a very high signal with the initial dilution but then the sensitivity tails off very rapidly. Diluting and coating the hexon in water alone slightly increases sensitivity but reduces strength; coating and diluting in the presence of a small amount of stabilising protein (such as BSA) seems to increase sensitivity for antigen coating significantly (although too much BSA can block high concentrations of hexon).

    This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: 0.09% Sodium azide
    Constituents: 2.19% Sodium chloride, 0.28% Bis-Tris propane

関連情報

  • 別名
    • Hexon protein
    • Late protein 2
    • PII
  • 関連性Hexon is one of the major structural components of the capsid, an icosahedral structure that envelops and protects the genetic material of adenoviruses. There are 240 hexon capsomeres per capsid and each capsomere is made out of a homotrimer of the hexon protein (> 900 residues). Human adenovirus are recognised as being an important cause of respiratory illnesses, conjunctivitis, gastroenteritis and, more recently, as a potential cause of sexually transmitted diseases. Adenovirus vectors, particularly those based on Ad5, have shown potential for the development of gene therapy protocols and clinical use.

Natural Adenovirus Type 5 hexon protein 画像

  • Lane 1: Ladder
    Lane 2: Ad5
    Lane 3: Ad5 crude prep
    Lane 4: Ad5 prep after filtration
    Lane 5: Hybrid purified Hexon

Natural Adenovirus Type 5 hexon protein (ab123995) 使用論文

ab123995 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123995.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"