Reacts with Atrial Natriuretic Polypeptide (human) 100% and Atrial Natriuretic Polypeptide (rat) ~ 60%
Does not cross react with ACTH, Calcitonin (salmon), PTH (human 1-84) or Vasoactive Intestinal Peptide
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Immunocytochemistry/ Immunofluorescence - Anti-Natriuretic peptides A antibody (ab40791)
ICC/IF image of ab40791 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40791 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.