Anti-Natriuretic Peptide Receptor C 抗体 (ab37617)

製品の概要

  • 製品名
    Anti-Natriuretic Peptide Receptor C antibody
    Natriuretic Peptide Receptor C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Natriuretic Peptide Receptor C
  • アプリケーション
    適用あり: ICC/IF, ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Natriuretic Peptide Receptor C aa 67-97 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: HL-60 cell lysate IHC-P: human breast carcinoma and kidney tissues. ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
  • 配列類似性
    Belongs to the ANF receptor family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Natriuretic Peptide Receptor C with ab37617. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-Natriuretic Peptide Receptor C antibody (ab37617) at 1/1000 dilution + HL-60 cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 60 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37617 staining Natriuretic Peptide Receptor C in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS  for 30 minutes. Samples were incubated with primary antibody (1/200) for 2 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit (1/1000) was used as the secondary antibody.

  • Anti-Natriuretic Peptide Receptor C antibody (ab37617) at 1/1000 dilution + HL-60 cell lysate at 35 µg/ml

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37617, at a 1/50 dliution staining human breast carcinoma by immunohistochemistry, formalin fixed paraffin embedded tissue, followed by AEC staining.

参考文献

This product has been referenced in:
  • Zhao F  et al. Molecular subgroups of adult medulloblastoma: a long-term single-institution study. Neuro Oncol 18:982-90 (2016). IHC-P ; Human . Read more (PubMed: 27106407) »
  • Pham CD  et al. Differential Immune Microenvironments and Response to Immune Checkpoint Blockade among Molecular Subtypes of Murine Medulloblastoma. Clin Cancer Res 22:582-95 (2016). IHC-P ; Mouse . Read more (PubMed: 26405194) »

See all 6 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Jul 23 2014

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