Anti-Natriuretic Peptide Receptor B 抗体 (ab55724)


  • 製品名
    Anti-Natriuretic Peptide Receptor B antibody
    Natriuretic Peptide Receptor B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Natriuretic Peptide Receptor B
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: AKNDHYRTLV RTGPSAPKLG EFVVTLHGHF NWTARAALLY LDARTDDRPH YFTIEGVFEA LQGSNLSVQH QVYAREPGGP EQATHFIRAN GRIVYICGPL , corresponding to amino acids 131-231 of Human Natriuretic Peptide Receptor B

  • ポジティブ・コントロール
    • ICC/IF: SKNSH cells.



Our Abpromise guarantee covers the use of ab55724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • 機能
    Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.
  • 関連疾患
    Defects in NPR2 are the cause of acromesomelic dysplasia Maroteaux type (AMDM) [MIM:602875]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers). AMDM is an autosomal recessive form characterized by axial skeletal involvement with wedging of vertebral bodies. In AMDM all skeletal elements are present but show abnormal rates of linear growth.
  • 配列類似性
    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation of the protein kinase-like domain is required for full activation by CNP.
  • 細胞内局在
  • Information by UniProt
  • 参照データベース
  • 別名
    • AMDM antibody
    • ANP-B antibody
    • ANPB antibody
    • ANPR-B antibody
    • ANPRB antibody
    • ANPRB_HUMAN antibody
    • Atrial natriuretic peptide B type receptor antibody
    • Atrial natriuretic peptide receptor 2 antibody
    • Atrial natriuretic peptide receptor type B antibody
    • Atrionatriuretic peptide receptor B antibody
    • GC-B antibody
    • GCB antibody
    • Guanylate cyclase B antibody
    • GUC 2B antibody
    • GUC2B antibody
    • GUCY2B antibody
    • Natriuretic peptide receptor B antibody
    • Natriuretic peptide receptor B/guanylate cyclase B antibody
    • NPR-B antibody
    • Npr2 antibody
    • NPRB antibody
    • NPRBi antibody
    see all

Anti-Natriuretic Peptide Receptor B antibody 画像

  • ICC/IF image of ab55724 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55724, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43um..

  • Natriuretic Peptide Receptor B antibody (ab55724) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab55724 Natriuretic Peptide Receptor B antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab55724 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SH-SY5Y cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Natriuretic Peptide Receptor B antibody (ab55724) 使用論文

ab55724 has not yet been referenced specifically in any publications.

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