Anti-Natriuretic Peptide Receptor A 抗体 (ab14356)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 118 kDa.Can be blocked with Human Natriuretic Peptide Receptor A peptide (ab28437).
IHC-P 1/4000.
ICC 1/200.
ICC/IF 1/300.
Flow Cyt 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/300.

ターゲット情報

  • 機能
    Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
  • 配列類似性
    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation of the protein kinase-like domain is required for full activation by ANP.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANP-A antibody
    • ANPa antibody
    • ANPR-A antibody
    • ANPRA antibody
    • ANPRA_HUMAN antibody
    • Atrial natriuretic peptide A type receptor antibody
    • Atrial natriuretic peptide receptor 1 antibody
    • Atrial natriuretic peptide receptor A antibody
    • Atrial natriuretic peptide receptor type A antibody
    • Atrionatriuretic peptide receptor A antibody
    • GC A antibody
    • GC-A antibody
    • Guanylate cyclase A antibody
    • Guanylate cyclase antibody
    • GUC2A antibody
    • GUCY2A antibody
    • Natriuretic peptide A type receptor antibody
    • Natriuretic peptide receptor A/guanylate cyclase A antibody
    • NPR-A antibody
    • NPR1 antibody
    • NPRA antibody
    see all

画像

  • ICC/IF image of ab14356 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14356, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • You H & Laychock SG Long-term treatment with atrial natriuretic peptide inhibits ATP production and insulin secretion in rat pancreatic islets. Am J Physiol Endocrinol Metab 300:E435-44 (2011). WB ; Rat . Read more (PubMed: 20959527) »
  • Matera MG  et al. Epithelium integrity is crucial for the relaxant activity of brain natriuretic peptide in human isolated bronchi. Br J Pharmacol 163:1740-54 (2011). Read more (PubMed: 21410689) »

See all 8 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Human Cell (HEK293)
Permeabilization
No
Gating Strategy
viability and PE
Specification
HEK293
Preparation
Cell harvesting/tissue preparation method: FACS buffer
Sample buffer: PBS with 1% FBS
Fixation
None
Username

Abcam user community

Verified customer

投稿 Nov 23 2015

Thank you for your enquiry which we have received through Labome.

For more information regarding ab14356 Natriuretic Peptide Receptor A antibody, I can recommend to review the online datasheet. This will contain all the information we have r...

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I received your voicemail and will give you a call. The authors of the recent Neuroscience paper have not replied to me. I examined the original reference: Hirsch JR et al. Cellular localization, membrane distribution, and possible function of guany...

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Thank you for getting back to me. I am pleased that you have published your data. Thank you very much for getting back to me. I have added this publication to the references specific for this antibody.

I'm sorry to hear you are having a problem with ab14356. Typically, an entirely black blot would mean that there is not enough blocking or far too much protein or antibody. A recent reviewer suggested that the antibody worked well although they did...

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Thank you for your enquiry. I have found two papers that use membrane loading controls: 1. BiP, found on ER: www.jbc.org/cgi/content/full/278/36/34725 We have several antiBiP antibodies; I suggest ab2902 http://www.abcam.com/index.html?d...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human aortic endothelial cells)
Loading amount
20 µg
Specification
human aortic endothelial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Brian Van Ness

Verified customer

投稿 Jul 17 2006

Thank you for your enquiry. I am pleased to report that I have clarified this issue. The immunogen that was used to raise Rabbit polyclonal to Natriuretic Peptide Receptor A (ab14356) was Natriuretic Peptide Receptor A peptide (294-308) (ab28437)...

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Thank you for your patience. The originator of ab14356 has told me that the antibody was tested with the corresponding antigen for which it was made for. I'm afraid that I'm unable to obtain any more information at this time. Rat kidney membrane sample...

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Thank you very much for your patience and for the details that you have provided. I'm currently working with the originator of this antibody and am waiting for some more information from them - in particular, what sort of lysate was used to charact...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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