Recombinant Streptolysin O (Hemolytic streptococcus) protein (ab63978)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant Streptolysin O (Hemolytic streptococcus) protein
Streptolysin O (Hemolytic streptococcus) タンパク質・ペプチド 製品一覧 -
生理活性
Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37? for 30 minutes.
The specific activity is as high as 1,200,000- 2,000,000 hemolytic units (HU) /mg.
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精製度
> 98 % SDS-PAGE.
Affinity-purified with Ni colum followed by gel-filtration -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Streptococcus -
配列
MGGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSR NKQN TANTETTTTN EQPKPESSEL TTEKAGQKMD DMLNSNDMIK LAPKEMPLES AEKEEKKSED NKKSEEDHTE EINDKIYSLN YNELEVLAKN GETIENFVPK EGVKKADKFI VIERKKKNIN TTPVDISIID SVTDRTYPAA LQLANKGFTE NKPDAVVTKR NPQKIHIDLP GMGDKATVEV NDPTYANVST AIDNLVNQWH DNYSGGNTLP ARTQYTESMV YSKSQIEAAL NVNSKILDGT LGIDFKSISK GEKKVMIAAY KQIFYTVSAN LPNNPADVFD KSVTLKELQR KGVSNEAPPL FVSNVAYGRT VFVKLETSSK SNDVEAAFSA ALKGTDVKTN GKYSDILENS SFTAVVLGGD AAEHNKVVTK DFDVIRNVIK DNATFSRKNP AYPISYTSVF LKNNKIAGVN NRSEYVETTS TEYTSGKINL SHQGAYVAQY EILWDEINYD DKGKEVITKR RWDNNWYSKT SPFSTVIPLG ANSRNIRIMA RECTGLAWEW WRKVIDERDV KLSKEINVNI SGSTLSPYGS ITYK -
領域
37 to 574 -
タグ
His tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab63978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Information about health hazards:
Target: Cholesterol on human and animal cell membrane
Health Hazards: May be fatal if enters bloodstream.
LD50-Lethal dose (50 percent kill) intravenous,
Rabbit, 1500 ng/kg (Ref :PHTHDT Pharmacology and Therapeutics. (Pergamon Press Ltd.,Headington Hill Hall, Oxford OX3 0BW, UK) Vol.(Issue) 11, Page 661,1981 )
Guinea pig, 12 ug/kg (Ref: BICMBE Biochimie. (SPPIF, B.P.22, F-41353 Vineuil, France, Vol.(Issue)55,Page 1187, 1973)
Toxicity is much less when introduced via other routes of entry like interdermal injection.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 6
Constituents: 49% Water, 0.015% DTT, 50% Glycerol (glycerin, glycerine), 0.1% Streptolysin, x1 Phosphate Buffered SalineThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Streptolysin O
- Streptolysin O (Thiol-activated cytolysin) slo
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関連性
Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab63978 は 5 報の論文で使用されています。
- Wang F et al. Interferon Gamma Induces Reversible Metabolic Reprogramming of M1 Macrophages to Sustain Cell Viability and Pro-Inflammatory Activity. EBioMedicine 30:303-316 (2018). PubMed: 29463472
- Blanca Ramírez M et al. GTP binding regulates cellular localization of Parkinson's disease-associated LRRK2. Hum Mol Genet 26:2747-2767 (2017). PubMed: 28453723
- Siau A et al. Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. Sci Rep 6:31055 (2016). PubMed: 27503796
- Lee YK et al. Modeling of Friedreich ataxia-related iron overloading cardiomyopathy using patient-specific-induced pluripotent stem cells. Pflugers Arch 466:1831-44 (2014). Functional Studies . PubMed: 24327207
- Gao N & Lehrman MA Mannose-6-phosphate: a regulator of LLO destruction. Methods Mol Biol 1022:277-82 (2013). PubMed: 23765668