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Our Abpromise guarantee covers the use of ab76842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/150. (see Abreview)|
|IHC-Fr||1/50 - 1/200.|
|WB||1/100 - 1/1000. Predicted molecular weight: 33 kDa.|
ab76842 staining FLJ37478 in CHO cells transfected with mouse Nat8l cDNA by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/150 in 1% BSA in PBS and incubated with the sample for 1 hour at 25°C. The secondary antibody was Cy2® conjugated goat anti-rabbit polyclonal, diluted 1/200.
This image is courtesy of an anonymous AbreveiwBlocking STep: 3% Milk for 30 minutes at 22 ��C
ab76842 has not yet been referenced specifically in any publications.