製品の概要

  • 製品名Anti-NASP antibody
    NASP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NASP
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human NASP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Testis tissue lysate as well as the following whole cell lysates: HeLa; HEK293; U20S and HeLa Nuclear extract.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 138 kDa (predicted molecular weight: 85 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
  • 組織特異性Isoform 1 is testis- and sperm-specific.
  • 配列類似性Belongs to the NASP family.
    Contains 3 TPR repeats.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5033430J04Rik antibody
    • AI131596 antibody
    • AI317140 antibody
    • D4Ertd767e antibody
    • DKFZp547F162 antibody
    • Epcs32 antibody
    • FLB7527 antibody
    • FLJ31599 antibody
    • FLJ35510 antibody
    • MGC19722 antibody
    • MGC20372 antibody
    • MGC2297 antibody
    • MGC93869 antibody
    • NASP antibody
    • NASP_HUMAN antibody
    • nuclear autoantigenic sperm protein (histone binding) antibody
    • Nuclear autoantigenic sperm protein antibody
    • PRO1999 antibody
    • RP23 233B9.9 antibody
    see all

Anti-NASP antibody 画像

  • All lanes : Anti-NASP antibody (ab104233) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 138 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab104233 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa, Hek293 and HepG2 cells at Xµg/ml.

Anti-NASP antibody (ab104233) 使用論文

ab104233 has not yet been referenced specifically in any publications.

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