製品の概要

  • 製品名
    Anti-NARS antibody [NARS2G5]
    NARS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NARS2G5] to NARS
  • アプリケーション
    適用あり: WB, IP, ICC, Dot blotmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment from Human NARS.

  • ポジティブ・コントロール
    • HT-1080 cells and whole cell lysate, NIH 3T3 whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab53473 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    NARS2G5
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use at 10 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 配列類似性
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3010001M15Rik antibody
    • AA960128 antibody
    • AsnRS antibody
    • ASNS antibody
    • Asparagine tRNA ligase 1, cytoplasmic antibody
    • Asparagine tRNA ligase antibody
    • Asparagine--tRNA ligase antibody
    • Asparaginyl tRNA synthetase antibody
    • Asparaginyl-tRNA synthetase antibody
    • C78150 antibody
    • cytoplasmic antibody
    • EC 6.1.1.22 antibody
    • LRRGT00113 antibody
    • MGC116236 antibody
    • Nars antibody
    • NARS1 antibody
    • NRS antibody
    • SYNC_HUMAN antibody
    see all

画像

  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
  • ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

参考文献

ab53473 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab53473.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録