製品の概要

  • 製品名
    Anti-NARS antibody [NARS2G5]
    NARS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NARS2G5] to NARS
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, IP, ICC, Dot blotmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment from Human NARS.

  • ポジティブ･コントロール
    • HT-1080 cells and whole cell lysate, NIH 3T3 whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項（精製）
    ab53473 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    NARS2G5
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use at 10 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 配列類似性
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 3010001M15Rik antibody
      • AA960128 antibody
      • AsnRS antibody
      • ASNS antibody
      • Asparagine tRNA ligase 1, cytoplasmic antibody
      • Asparagine tRNA ligase antibody
      • Asparagine--tRNA ligase antibody
      • Asparaginyl tRNA synthetase antibody
      • Asparaginyl-tRNA synthetase antibody
      • C78150 antibody
      • cytoplasmic antibody
      • EC 6.1.1.22 antibody
      • LRRGT00113 antibody
      • MGC116236 antibody
      • Nars antibody
      • NARS1 antibody
      • NRS antibody
      • SYNC_HUMAN antibody
      see all

    画像

    • Western blot - Anti-NARS antibody [NARS2G5] (ab53473)
      Western blot - Anti-NARS antibody [NARS2G5] (ab53473)
      Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 63 kDa
      Observed band size: 63 kDa

    • Immunoprecipitation - Anti-NARS antibody [NARS2G5] (ab53473)
      Immunoprecipitation - Anti-NARS antibody [NARS2G5] (ab53473)
      ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
    • Immunocytochemistry - Anti-NARS antibody [NARS2G5] (ab53473)
      Immunocytochemistry - Anti-NARS antibody [NARS2G5] (ab53473)
      ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
    • Western blot - Anti-NARS antibody [NARS2G5] (ab53473)
      Western blot - Anti-NARS antibody [NARS2G5] (ab53473)
      Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 63 kDa
      Observed band size: 63 kDa

    参考文献

    ab53473 has not yet been referenced specifically in any publications.

    ab53473 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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