製品の概要

  • 製品名Anti-NARG1 antibody
    NARG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NARG1
  • アプリケーション適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide (Human) from an internal sequence.

  • ポジティブ・コントロール
    • Extracts from HUVEC cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
  • 組織特異性Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
  • 配列類似性Contains 8 TPR repeats.
  • 翻訳後修飾Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.
  • 細胞内局在Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5730450D16Rik antibody
    • 6330400I15 antibody
    • ASTBDN antibody
    • GA19 antibody
    • Gastric cancer antigen Ga19 antibody
    • MGC29428 antibody
    • mNAT1 antibody
    • N alpha acetyltransferase 15, NatA auxiliary subunit antibody
    • N terminal acetyltransferase antibody
    • N(alpha) acetyltransferase 15, NatA auxiliary subunit antibody
    • N-alpha-acetyltransferase 15 antibody
    • N-terminal acetyltransferase antibody
    • Naa15 antibody
    • NAA15_HUMAN antibody
    • NatA auxiliary subunit antibody
    • NATH antibody
    • NMDA receptor regulated 1 antibody
    • NMDA receptor regulated protein 1 antibody
    • NMDA receptor-regulated protein 1 antibody
    • Protein tubedown 1 antibody
    • Protein tubedown-1 antibody
    • Tbdn 1 antibody
    • Tbdn100 antibody
    • Transcriptional coactivator tubedown 100 antibody
    • tubedown-1 antibody
    see all

Anti-NARG1 antibody 画像

  • All lanes : Anti-NARG1 antibody (ab65107) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • ICC/IF image of ab65107 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65107, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NARG1 antibody (ab65107) 使用論文

ab65107 has not yet been referenced specifically in any publications.

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