Anti-NAPSIN A 抗体 [KCG1.1] (ab73021)

製品の概要

  • 製品名Anti-NAPSIN A antibody [KCG1.1]
    NAPSIN A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [KCG1.1] to NAPSIN A
  • 特異性Napsin A reactivity was determined immunohistochemically on formalin-fixed paraffin-embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). Napsin A was positive in 10/12 pulmonary adenocarcinomas. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive.
  • アプリケーション適用あり: Flow Cyt, ICC, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N terminus of human NAPSIN A

  • ポジティブ・コントロール
    • Lung Adenocarcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC 1/100.
ICC/IF 1/50. (customer data using A549 cells)
IHC-P 1/50 - 1/100. 30 minutes at room temperature in an ABC method. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining

ターゲット情報

  • 機能May be involved in processing of pneumocyte surfactant precursors.
  • 組織特異性Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
  • 配列類似性Belongs to the peptidase A1 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Asp 4 antibody
    • ASP4 antibody
    • Aspartyl protease 4 antibody
    • KAP antibody
    • Kdap antibody
    • Kidney derived aspartic protease like protein antibody
    • NAP1 antibody
    • NAPA antibody
    • Napsa antibody
    • NAPSA_HUMAN antibody
    • Napsin 1 antibody
    • napsin A aspartic peptidase antibody
    • Napsin A precursor antibody
    • Napsin-1 antibody
    • Napsin-A antibody
    • Pronapsin A antibody
    • SNAPA antibody
    • TA01/TA02 antibody
    see all

Anti-NAPSIN A antibody [KCG1.1] 画像

  • ab73021, at 1 in 50 dilution, staining NAPSIN A in Human lung adenocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • Overlay histogram showing A549 cells stained with ab73021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73021, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab73021 staining NAPSIN A in cells from human broncho-alveolar lavage by Immunocytochemistry.
    Cells were fixed in formaldehyde, permeabilized using Triton, blocked with 5% normal horse serum for 1 hour at room temperature and then incubated with ab73021 at a 1/100 dilution for 16 hours. The secondary used was a biotin conjugated horse anti-mouse polyclonal used at a 1/300 dilution.

    See Abreview

Anti-NAPSIN A antibody [KCG1.1] (ab73021) 使用論文

ab73021 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (cells from broncho-alveolar lavage)
Specification cells from broncho-alveolar lavage
Fixative Formaldehyde
Permeabilization Yes - PBS triton 0.25% 10 min
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jan 16 2012

Merci pour votre appel. Je viens de trouver l'information. Je peux donc confirmer que le ab73021 lot GR4651 est à une concentration de 100 ug/ml. Je vous souhaite une bonne soirée et bon succès pour l'expérience de demain!

Code de remise : **** Date d’expiration : JJ MM AAAA Merci de nous avoir contactés de nouveau. Ma collègue du service clientèle m'a informé que vous aviez décidé de participer à notre offre et tester ab73021 en ICC. Le code de réduction correspond...

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Merci de votre intérêt pour ab73021. A ma connaissance, cet anticorps n’a pas encore été testé en ICC et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab73021 en ICC, il me sera possi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"