製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NAP1L4
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAP1L4.

    (Peptide available as ab22419.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human whole cell lysates: U2OS; HeLa; A431.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 43 kDa).
IP Use at an assay dependent concentration. PubMed: 23637612

ターゲット情報

  • 関連性
    This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • NAP2 antibody
    • Nucleosome assembly protein 1-like 4 antibody
    • Nucleosome assembly protein 2 antibody

画像

  • Lanes 1-2 : Anti-NAP1L4 antibody (ab21631) at 1/1000 dilution
    Lanes 3-4 : Anti-NAP1L4 antibody (ab21631) at 1/500 dilution
    Lanes 5-6 : Anti-NAP1L4 antibody (ab21631) at 1/250 dilution

    Lanes 1 & 3 & 5 : U2OS whole cell lysate
    Lanes 2 & 4 & 6 : U2OS whole cell lysate, treated with ionizing radiation

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity), 64 kDa (possible cross reactivity)



    For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60 mm dish and added to 75 µl of 2X Laemmli buffer. 20 µl of these samples were loaded into each lane. ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.

  • All lanes : Anti-NAP1L4 antibody (ab21631) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 30 kDa (possible cleavage fragment), 30 kDa (possible cross reactivity)



    ab21631 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L4, which has been shown to migrate at approximately 50-55 kDa (Rodriquez, et al., J. of Cell. Biochemistry 93:398-408). It also detects a cross-reacting band at 30 kDa.

参考文献

This product has been referenced in:
  • Cho I  et al. ATP-dependent chromatin remodeling by Cockayne syndrome protein B and NAP1-like histone chaperones is required for efficient transcription-coupled DNA repair. PLoS Genet 9:e1003407 (2013). IP ; Human . Read more (PubMed: 23637612) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Sep 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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