Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 10 µg/ml.
||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).
Anti-NAP1L3 antibody 画像
Western blot - NAP1L3 antibody (ab70205)
All lanes :
Anti-NAP1L3 antibody (ab70205) at 1/500 dilutionLane 1 :
NAP1L3 transfected 293T cell lysateLane 2 :
Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutionPredicted band size :
58 kDaObserved band size :
70 kDa (why is the actual band size different from the predicted?
)Additional bands at :
75 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - NAP1L3 antibody (ab70205)
ab70205 at 10µg/ml staining NAP1L3 in Hela cells by Immunocytochemistry/Immunofluorescence.
Anti-NAP1L3 antibody (ab70205) 使用論文
has not yet been referenced specifically in any publications.
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