Rabbit polyclonal to NAP1 (C12orf30)
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human NAP1 (C12orf30).
(Peptide available as
This antibody gave a positive signal in HeLa and HepG2 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 112 kDa).
Use a concentration of 10 µg/ml.
Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression.
Belongs to the MDM20/NAA25 family.
Contains 4 TPR repeats.
Information by UniProt
There are 2 isoforms produced by alternative splicing.
Western blot - NAP1 (C12orf30) antibody (ab79490)
All lanes :
Anti-NAP1 (C12orf30) antibody (ab79490) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
Additional bands at:
46 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - NAP1 (C12orf30) antibody (ab79490)
ICC/IF image of ab79490 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79490, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 10µg/ml.
has not yet been referenced specifically in any publications.
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