Anti-Nanos3 抗体 (ab70001)
Key features and details
- Rabbit polyclonal to Nanos3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nanos3 antibody -
製品の詳細
Rabbit polyclonal to Nanos3 -
由来種
Rabbit -
特異性
ab70001 does not cross-react with either Nanos1 or Nanos2. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic 16 amino acid peptide near the carboxy terminus of the human Nanos3 (GenBank accession no. P60323).
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ポジティブ・コントロール
- IHC-P: Human brain tissue. WB: Human brain tissue lysate. ICC/IF: Human brain cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70001の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2.5 µg/ml. |
ターゲット情報
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機能
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis. -
組織特異性
Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids. -
配列類似性
Belongs to the nanos family.
Contains 1 nanos-type zinc finger. -
発生段階
Fetal ovary and fetal testis (at protein level). -
ドメイン
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm > P-body. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body. - Information by UniProt
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参照データベース
- Entrez Gene: 342977 Human
- Entrez Gene: 244551 Mouse
- Entrez Gene: 288909 Rat
- Omim: 608229 Human
- SwissProt: P60323 Human
- SwissProt: P60324 Mouse
- Unigene: 127982 Human
- Unigene: 216740 Mouse
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別名
- NANO3_HUMAN antibody
- Nanos homolog 3 antibody
- NANOS3 antibody
see all
画像
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ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
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Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Immunofluorescence of Nanos3 in Human Brain cells using ab70001 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab70001 は 12 報の論文で使用されています。
- Ma Z et al. Activation of embryonic/germ cell-like axis links poor outcomes of gliomas. Cancer Cell Int 22:371 (2022). PubMed: 36435765
- Cho J et al. Vascular remodeling by placenta-derived mesenchymal stem cells restores ovarian function in ovariectomized rat model via the VEGF pathway. Lab Invest 101:304-317 (2021). PubMed: 33303971
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Zhang F et al. Nanos3, a cancer-germline gene, promotes cell proliferation, migration, chemoresistance, and invasion of human glioblastoma. Cancer Cell Int 20:197 (2020). WB . PubMed: 32508533
- Zhao J et al. Expression pattern and potential role of Nanos3 in regulating testosterone biosynthesis in Leydig cells of sheep. Theriogenology 154:31-42 (2020). PubMed: 32474303