製品の概要

  • 製品名Anti-NANOS2 antibody
    NANOS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NANOS2
  • アプリケーション適用あり: IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    KLH conjugated synthetic peptide selected from the C terminal region of human NANOS2.

  • ポジティブ・コントロール
    • Human testis tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab76568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/50.
ELISA 1/1000.

ターゲット情報

  • 機能Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.
  • 組織特異性Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).
  • 配列類似性Belongs to the nanos family.
    Contains 1 nanos-type zinc finger.
  • 発生段階Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).
  • ドメインThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
  • 細胞内局在Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NANO2_HUMAN antibody
    • Nanos homolog 2 antibody
    • Nanos homolog 2 Drosophila antibody
    • NANOS2 antibody
    • NOS-2 antibody
    • NOS2 antibody
    see all

Anti-NANOS2 antibody 画像

  • Formalin fixed and paraffin embedded human testis tissue stained with ab76568 at 1/10 dilution, followed by treatment with a peroxidase conjugated secondary antibody and DAB staining.

Anti-NANOS2 antibody (ab76568) 使用論文

This product has been referenced in:
  • Santos Nassif Lacerda SM  et al. Phenotypic characterization and in vitro propagation and transplantation of the Nile tilapia (Oreochromis niloticus) spermatogonial stem cells. Gen Comp Endocrinol 192:95-106 (2013). Read more (PubMed: 23792279) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (testis)
Permeabilization No
Specification testis
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
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投稿 Apr 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"