製品の概要

  • 製品名Anti-NANOS2 antibody
    NANOS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NANOS2
  • 特異性ab70000 does not cross-react with either Nanos1 or Nanos3.
  • アプリケーション適用あり: WBmore details
    適用なし: IHC
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human NANOS2 (C terminal). 13 amino acid peptide near the carboxy terminus of the human NANOS2.

  • ポジティブ・コントロール
    • SK-N-SH cell lysate, mouse brain, mouse spleen, A20, 3T3(NIH)
  • 特記事項

    Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
  • 追加情報Is unsuitable for IHC.
  • ターゲット情報

    • 機能Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.
    • 組織特異性Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).
    • 配列類似性Belongs to the nanos family.
      Contains 1 nanos-type zinc finger.
    • 発生段階Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).
    • ドメインThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
    • 細胞内局在Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NANO2_HUMAN antibody
      • Nanos homolog 2 antibody
      • Nanos homolog 2 Drosophila antibody
      • NANOS2 antibody
      • NOS-2 antibody
      • NOS2 antibody
      see all

    Anti-NANOS2 antibody 画像

    • All lanes : Anti-NANOS2 antibody (ab70000)

      Lane 1 : Mouse Brain
      Lane 2 : Mouse Spleen
      Lane 3 : A20
      Lane 4 : 3T3(NIH)


      Predicted band size : 15 kDa
    • Anti-NANOS2 antibody (ab70000) at 2 µg/ml + SK-N-SH cell lysate at 15 µg

      Predicted band size : 15 kDa
      Observed band size : 15 kDa

    Anti-NANOS2 antibody (ab70000) 使用論文

    This product has been referenced in:
    • Kusz KM  et al. The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction. Mol Hum Reprod 15:165-71 (2009). IHC-P, WB ; Human . Read more (PubMed: 19168545) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Testis, embryonic day 15.5)
    Permeabilization Yes - 0.1% Triton X-100 in PBS
    Specification Testis, embryonic day 15.5
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Mr. Bryan Niedenberger

    Verified customer

    投稿 Apr 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"