Previous batches of ab70000 have been successfully used in IHC-P on testis tissue sections (please see Kusz KM et al., 2009). However, when tested on human brain tissue sections, ab70000 gave negative results in IHC-P (data not shown). We do not guarantee the current batch of ab70000 to work in IHC.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
追加情報Is unsuitable for IHC.
機能Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.
組織特異性Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).
配列類似性Belongs to the nanos family. Contains 1 nanos-type zinc finger.
発生段階Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).
ドメインThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
細胞内局在Cytoplasm. Cytoplasm > P-body. Cytoplasm > perinuclear region. Localizes at P-bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.
Lane 1 : Mouse Brain Lane 2 : Mouse Spleen Lane 3 : A20 Lane 4 : 3T3(NIH)
Predicted band size : 15 kDa
Western blot - NANOS2 antibody (ab70000)
Anti-NANOS2 antibody (ab70000) at 2 µg/ml + SK-N-SH cell lysate at 15 µg
Predicted band size : 15 kDa Observed band size : 15 kDa
Anti-NANOS2 antibody (ab70000) 使用論文
This product has been referenced in:
Kusz KM et al. The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction. Mol Hum Reprod15:165-71 (2009).
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