Anti-Nanos Homologue 1 (NANOS1) 抗体 (ab83417)

製品の概要

  • 製品名Anti-Nanos Homologue 1 (NANOS1) antibody
    Nanos Homologue 1 (NANOS1) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nanos Homologue 1 (NANOS1)
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Pig
  • 免疫原

    Synthetic peptide: DDDDDSDEPG SRGRYLGSAL ELRALELCAG PAEAGLLEER FAELSPFAGR , corresponding to a region within internal sequence amino acids 108 - 157 of Human Nanos Homologue 1 (NANOS1) (NP_955631)

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
  • 組織特異性Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
  • 配列類似性Belongs to the nanos family.
    Contains 1 nanos-type zinc finger.
  • 発生段階Fetal ovary and fetal testis (at protein level).
  • ドメインThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
    The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
  • 細胞内局在Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC Rep1a antibody
    • EC_Rep1a antibody
    • NANO1_HUMAN antibody
    • NANOS 1 antibody
    • Nanos homolog 1 (Drosophila) antibody
    • Nanos homolog 1 antibody
    • NANOS1 antibody
    • Nos 1 antibody
    • NOS-1 antibody
    • Nos1 antibody
    • OTTHUMP00000046853 antibody
    • Rep 1a antibody
    • Rep1a antibody
    see all

Anti-Nanos Homologue 1 (NANOS1) antibody 画像

  • Anti-Nanos Homologue 1 (NANOS1) antibody (ab83417) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

Anti-Nanos Homologue 1 (NANOS1) antibody (ab83417) 使用論文

ab83417 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"