製品の概要

  • 製品名Anti-Nanos Homologue 1 (NANOS1) antibody
    Nanos Homologue 1 (NANOS1) 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Nanos Homologue 1 (NANOS1)
  • 特異性This antibody is expected to recognise the longer isoform 1 only, not isoform 2.
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    SARDGPPGKKLR

    , corresponding to amino acids 281-292 of Human Nanos Homologue 1 (NANOS1)

  • ポジティブ・コントロール
    • Human testes lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
IHC-P
  • 追加情報ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
    • 組織特異性Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
    • 配列類似性Belongs to the nanos family.
      Contains 1 nanos-type zinc finger.
    • 発生段階Fetal ovary and fetal testis (at protein level).
    • ドメインThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
      The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
    • 細胞内局在Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC Rep1a antibody
      • EC_Rep1a antibody
      • NANO1_HUMAN antibody
      • NANOS 1 antibody
      • Nanos homolog 1 (Drosophila) antibody
      • Nanos homolog 1 antibody
      • NANOS1 antibody
      • Nos 1 antibody
      • NOS-1 antibody
      • Nos1 antibody
      • OTTHUMP00000046853 antibody
      • Rep 1a antibody
      • Rep1a antibody
      see all

    Anti-Nanos Homologue 1 (NANOS1) antibody 画像

    • Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163) at 0.3 µg/ml (incubated for 1 hour) + Human Testis lysate in RIPA buffer. at 35 µg
      Developed using the ECL technique

      Predicted band size : 30 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163) 使用論文

    This product has been referenced in:
    • Bonnomet A  et al. The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. Oncogene 27:3692-9 (2008). IHC-P ; Human . Read more (PubMed: 18223680) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"