製品の概要

  • 製品名Anti-Nanog antibody [PR-3F6]
    Nanog 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PR-3F6] to Nanog
  • アプリケーション適用あり: Indirect ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human Nanog expressed in E. coli and refolded to soluble form.

  • ポジティブ・コントロール
    • I-ELISA: immobilized Nanog immunogen WB: Nanog recombinant protein, HEK-293-Nanog transfectant cell lysate, NTERA-2 cell lysate, 2102 cell lysate
  • 特記事項This product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA 1/1000.
WB 1/1000. Predicted molecular weight: 35 kDa.

ターゲット情報

  • 機能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 組織特異性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 配列類似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 発生段階Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody [PR-3F6] 画像

  • All lanes : Anti-Nanog antibody [PR-3F6] (ab95335) at 1 µg/ml

    Lane 1 : Nanog recombinant protein at 0.1 µg
    Lane 2 : Untransfected HEK-293 cell lysate at 20 µg
    Lane 3 : HEK-293-Nanog transfectant cell lysate at 20 µg
    Lane 4 : NTERA-2 cell lysate at 20 µg
    Lane 5 : 2102 cell lysate at 20 µg


    Predicted band size : 35 kDa
  • Indirect ELISA detection of immobilized Nanog immunogen with titrated amounts of ab95335. No binding observed to irrelevant (SOX2) protein.

Anti-Nanog antibody [PR-3F6] (ab95335) 使用論文

ab95335 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"