製品の概要

  • 製品名Anti-Nanog antibody
    Nanog 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Nanog
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Cynomolgus Monkey
  • 免疫原

    Recombinant fragment: TRTVFSSTQL CVLNDRFQRQ KYLSLQQMQE LSNILNLSYK QVKTWFQNQR MKSKRWQKNN WPKNSNGVTQ KASAPTYPSL YSSYHQGCLV NPTGNLPM, corresponding to amino acids 98-195 of Human Nanog (NP_079141) with a proprietary tag.

  • ポジティブ・コントロール
    • HeLa nuclear extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Nanog is approximately 0.3ng/ml when used as a capture antibody.

ターゲット情報

  • 機能Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • 組織特異性Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • 配列類似性Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 発生段階Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody 画像

Anti-Nanog antibody (ab89500) 使用論文

This product has been referenced in:
  • Huang ZJ  et al. Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells. Mol Med Rep 11:1647-54 (2015). WB ; Human . Read more (PubMed: 25405855) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"