Anti-Nanog 抗体 (ab77095)
Key features and details
- Goat polyclonal to Nanog
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nanog antibody
Nanog 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Nanog -
由来種
Goat -
特異性
We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog, Pig非交差種: Mouse -
免疫原
Synthetic peptide:
QNQRMKSKRWQKNN
by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog -
ポジティブ・コントロール
- Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77095の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples. |
特記事項 |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples. |
ターゲット情報
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機能
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
組織特異性
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
配列類似性
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
発生段階
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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別名
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
画像
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Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab77095 は 10 報の論文で使用されています。
- Recchia K et al. In vitro induced pluripotency from urine-derived cells in porcine. World J Stem Cells 14:231-244 (2022). PubMed: 35432738
- Trujillo CA et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759
- Pinto F et al. The Extracellular Small Leucine-Rich Proteoglycan Biglycan Is a Key Player in Gastric Cancer Aggressiveness. Cancers (Basel) 13:N/A (2021). PubMed: 33809543
- Sauer H et al. The nicotinamide phosphoribosyltransferase antagonist FK866 inhibits growth of prostate tumour spheroids and increases doxorubicin retention without changes in drug transporter and cancer stem cell protein expression. Clin Exp Pharmacol Physiol 48:422-434 (2021). PubMed: 33349973
- Kimura K et al. Canine induced pluripotent stem cell maintenance under feeder-free and chemically-defined conditions. Mol Reprod Dev 88:395-404 (2021). PubMed: 34010985
- Li Q et al. Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells. Cell Reprogram 22:82-89 (2020). PubMed: 32125888
- Chailangkarn T et al. A human neurodevelopmental model for Williams syndrome. Nature 536:338-43 (2016). PubMed: 27509850
- Lim J et al. Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors. Sci Rep 4:4361 (2014). ICC/IF ; Human . PubMed: 24618595
- Nishimura T et al. Generation of Functional Platelets from Canine Induced Pluripotent Stem Cells. Stem Cells Dev N/A:N/A (2013). PubMed: 23409943
- Montserrat N et al. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system. J Biol Chem 286:12417-28 (2011). ICC/IF . PubMed: 21285354