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A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2
Our Abpromise guarantee covers the use of ab70482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20331356|
|IHC-P||1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
ab70482 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with HIER buffer. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab70482 at 1/750 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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