The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.625 µg/ml.
Nalidixic acid (C12H12N2O3, systematic name 1-ethyl-7-methyl-4-oxo-[1,8]naphthyridine-3-carboxylic acid) is a quinolone antibiotic which inhibits DNA-gyrase. It is used in the treatment of urinary tract infections as it reaches high levels in the urine and is effective over the entire urinary pH range.
Anti-Nalidixic Acid antibody (ab58943) 使用論文
has not yet been referenced specifically in any publications.