リコンビナント
RabMAb

Anti-NAK/TBK1 (phospho S172) 抗体 [EPR2867(2)] (ab109272)

製品の概要

  • 製品名
    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)]
    NAK/TBK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172)
  • 特異性
    This antibody only detects NAK/TBK1 phosphorylated at serine 172.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NAK/TBK1 .
    (Peptide available as ab206124)

  • ポジティブ・コントロール
    • HeLa cell lysate, treated with Calyculin A.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124).
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for ICC.
  • ターゲット情報

    • 機能
      Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
    • 組織特異性
      Ubiquitous with higher expression in testis.
    • 配列類似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 2.7.11.1 antibody
      • FLJ11330 antibody
      • FTDALS4 antibody
      • NAK antibody
      • NF kappa B activating kinase antibody
      • NF kB activating kinase antibody
      • NF-kappa-B-activating kinase antibody
      • Serine/threonine protein kinase TBK 1 antibody
      • Serine/threonine protein kinase TBK1 antibody
      • Serine/threonine-protein kinase TBK1 antibody
      • T2K antibody
      • TANK binding kinase 1 antibody
      • TANK-binding kinase 1 antibody
      • TBK 1 antibody
      • Tbk1 antibody
      • TBK1_HUMAN antibody
      see all

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] 画像

    • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

      Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : NIH/3T3 (human cervix adenocarcinoma) treated with 10mM pervanadate for 30 minutes whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 84 kDa

      Blocking buffer and diluting buffer: 5% NFDM /TBST

    • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma) treated with Calyculin A whole cell lysate
      Lane 2 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 84 kDa
      Additional bands at : 84 kDa. We are unsure as to the identity of these extra bands.

      Blocking buffer and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) 使用論文

    This product has been referenced in:
    • Chen LT  et al. MSX1 Modulates RLR-Mediated Innate Antiviral Signaling by Facilitating Assembly of TBK1-Associated Complexes. J Immunol 197:199-207 (2016). Read more (PubMed: 27194789) »
    • Zhang Q  et al. Rho kinase II interference by small hairpin RNA ameliorates 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in mice. Mol Med Rep 14:4947-4956 (2016). Mouse . Read more (PubMed: 27840922) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (human colon lysates)
    Loading amount
    50 µg
    Specification
    human colon lysates
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 19 2012

    Thank you for your recent telephone call. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    It has taken a little time to obtain more information regarding the WB testing protocol and I appreciate ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録