製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NAIP
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAIP.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human liver tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).

ターゲット情報

  • 機能
    Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
  • 組織特異性
    Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
  • 配列類似性
    Contains 3 BIR repeats.
    Contains 1 NACHT domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Baculoviral IAP repeat containing 1 antibody
    • Baculoviral IAP repeat-containing protein 1 antibody
    • BIRC 1 antibody
    • BIRC1 antibody
    • BIRC1_HUMAN antibody
    • Birc1a antibody
    • FLJ42520 antibody
    • NAIP antibody
    • Naip1 antibody
    • Neuronal apoptosis inhibitory protein antibody
    • NLR family apoptosis inhibitory protein antibody
    • NLR family BIR domain containing 1 antibody
    • NLRB 1 antibody
    • NLRB1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1 antibody
    • Psi neuronal apoptosis inhibitory protein antibody
    • psiNAIP antibody
    • Similar to occludin antibody
    see all

画像

  • Anti-NAIP antibody (ab98020) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 160 kDa
    Observed band size : 160 kDa
    Additional bands at : 31 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab98020 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98020, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.

参考文献

This product has been referenced in:
  • Abadía-Molina F  et al. Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division. Sci Rep 7:39981 (2017). Read more (PubMed: 28059125) »

See 1 Publication for this product

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