製品の概要

  • 製品名Anti-NADPH oxidase 4 antibody
    NADPH oxidase 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NADPH oxidase 4
  • アプリケーション適用あり: Flow Cyt, ICC, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Cow, Monkey
  • 免疫原

    A synthetic peptide made to a region within residues 100-200 of the human NOX4 protein.

  • ポジティブ・コントロール
    • Human kidney tissue and lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/250. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.
  • 組織特異性Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
  • 配列類似性Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • 発生段階Expressed in fetal kidney and fetal liver.
  • 翻訳後修飾Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
  • 細胞内局在Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Kidney oxidase-1 antibody
    • Kidney superoxide-producing NADPH oxidase antibody
    • KOX 1 antibody
    • KOX antibody
    • Kox-1 antibody
    • NADPH antibody
    • NADPH oxidase 4 antibody
    • Nox4 antibody
    • NOX4_HUMAN antibody
    • Renal NAD(P)H-oxidase antibody
    • RENOX antibody
    see all

Anti-NADPH oxidase 4 antibody 画像

  • Anti-NADPH oxidase 4 antibody (ab60940) at 2 µg/ml + Human kidney lysate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : 50 kDa (possible non-specific binding).
  • Immunohistochemical staining of human kidney tissue with ab60940 (5µg/ml) staining proximal convoluted tubules of the kidney. Formalin fixed, paraffin section.
  • ICC/IF image of ab60940 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60940, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NADPH oxidase 4 antibody (ab60940) 使用論文

This product has been referenced in:
  • Ha SY  et al. NADPH Oxidase 1 and NADPH Oxidase 4 Have Opposite Prognostic Effects for Patients with Hepatocellular Carcinoma after Hepatectomy. Gut Liver 10:826-35 (2016). Read more (PubMed: 27282266) »
  • Kern G  et al. Tacrolimus increases nox4 expression in human renal fibroblasts and induces fibrosis-related genes by aberrant tgf-Beta receptor signalling. PLoS One 9:e96377 (2014). WB ; Human . Read more (PubMed: 24816588) »

See all 15 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (BEL-7402)
Permeabilization No
Specification BEL-7402
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Feb 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (EA. hy926)
Loading amount 10 µg
Specification EA. hy926
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 02 2013

Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount 30 µg
Specification Glioblastoma primary cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 07 2012

Thanks for your reply.
I'm happy to arrange ab8898 be shipped as a replacement for ab60940.
Please forward the PO number or Abcam order reference number associated with purchase of ab60940 so I can process your request.

Application Western blot
Sample Rat Tissue lysate - whole (Rat kidney tissue)
Loading amount 140 µg
Specification Rat kidney tissue
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 04 2012

Thank you for your enquiry and for providing the protocol details and western blotting image.
I agree this antibody is not working as expected. If you have purchased this antibody within the last 6 months I would be happy to offer a replacement of...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Cryosection-aorta)
Specification Cryosection-aorta
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 02 2012

Thank you for your reply.
Unfortunately we are unable to supply the peptide that was used to generate ab60940.
I am sorry that I could not help further in this matter and if there is anything else I can help you with, please let me know.

Thank you for contacting Abcam.

The immunogen used to generate ab60940 is a synthetic peptide made to a region within residues 100-200 of the human NOX4 protein (http://www.uniprot.org/uniprot/Q9NPH5).

Since the antibody is a polyclon...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
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Dr. Mahesh Shivananjappa

Verified customer

投稿 Jun 21 2012

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