Anti-NADH Dehydrogenase subunit 6 抗体 (ab81212)

製品の概要

  • 製品名
    Anti-NADH Dehydrogenase subunit 6 antibody
    NADH Dehydrogenase subunit 6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NADH Dehydrogenase subunit 6
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Pig, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 110-159 (DGVVVVVNFN SVGSWMIYEG EGSGLIREDP IGAGALYDYG RWLVVVTGWT) of Human NADH Dehydrogenase subunit 6 (NP_536854).

  • ポジティブ・コントロール
    • Human fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    NADH Dehydrogenase subunit 6 (MTND6) is 1 of the 7 mitochondrial DNA (mtDNA) encoded subunits (MTND1, MTND2, MTND3, MTND4L, MTND4, MTND5, MTND6) included among the approximately 41 polypeptides of respiratory Complex I. Complex I accepts electrons from NADH, transfers them to ubiquinone (Coenzyme Q10), and uses the energy released to pump protons across the mitochondria inner membrane. MTND6 has been proposed to be a component of the iron-protein fragment. The predicted polypeptide has a molecular weight of 18.6 kD. Its apparent MW on SDS-polyacrylamide gels (PAGE) using Tris-glycine buffer is 16.7 kD, and the apparent MW on urea-phosphate gels is also close to the predicted molecular weight.
  • 細胞内局在
    Mitochondrion membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • Mitochondrially encoded NADH dehydrogenase 6 antibody
    • MT ND6 antibody
    • mtND6 antibody
    • NADH dehydrogenase subunit 6 (complex I) antibody
    • NADH ubiquinone oxidoreductase chain 6 antibody
    • NADH Ubiquinone Oxidoreductase subunit ND6 antibody
    • NADH6 antibody
    • ND6 antibody
    see all

画像

  • Anti-NADH Dehydrogenase subunit 6 antibody (ab81212) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 19 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab81212 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81212, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Zhu G  et al. Acute effect of lactic acid on tumor-endothelial cell metabolic coupling in the tumor microenvironment. Oncol Lett 12:3478-3484 (2016). WB ; Human . Read more (PubMed: 27900024) »
  • Franz A  et al. Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression. Nat Commun 7:10612 (2016). Read more (PubMed: 26842564) »

See all 5 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Dec 22 2014

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (MDM)
Specification
MDM
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 27 2013


Upon reviewing your protocol, it is troubling to me that all 4 of these antibodies are not working. While it is conceivable that all four antibodies are not performing properly, typically when multiple antibodies are not working it is an issue wit...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole larval extract)
Loading amount
100 µg
Specification
Whole larval extract
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Nov 05 2012

Application
Western blot
Sample
Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount
26 µg
Specification
Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 21 2012

Thank you for your enquiry regarding ab81212 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

The protocol looks fine to me, however i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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